Quantitative Analysis of Steroid Autoradiograms,
نویسندگان
چکیده
منابع مشابه
Semi-automated, single-band peak-fitting analysis of hydroxyl radical nucleic acid footprint autoradiograms for the quantitative analysis of transitions.
Hydroxyl radical footprinting can probe the solvent accessibility of the ribose moiety of the individual nucleotides of DNA and RNA. Semi-automated analytical tools are presented for the quantitative analyses of nucleic acid footprint transitions in which processes such as folding or ligand binding are followed as a function of time or ligand concentration. Efficient quantitation of the intensi...
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15 صفحه اولA simplified method for computer analysis of autoradiograms from two-dimensional gels.
A simple method is described for computer analysis of a discrete number of spots on autoradiograms from two-dimensional gels. The method involves digitizing the density data on an autoradiograph with a rotating drum densitometer and displaying the data on a graphics computer terminal. The software allows the operator to select the boundaries of the spots to be analyzed from the terminal, then i...
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A nucleotide-by-nucleotide density fitting procedure has been successfully developed and has been applied to an analysis of a domain of the Tetrahymena ribozyme and a TBP-DNA interaction. This procedure is described in a paper that has been submitted for publication [Pastor, N., Weinstein, H., Jamison, E. & Brenowitz, M. A Detailed Interpretation of OH Radical Footprints in a TBP-DNA Complex Re...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1981
ISSN: 0022-1554,1551-5044
DOI: 10.1177/29.1a_suppl.7288157